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تغییرات miR146b متعاقب یک دوره مداخلات ورزشی مختلف در نوجوانان چاق یا دارای اضافه‌وزن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران

2 دانشیار گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان- اصفهان، ایران

3 دکتری پژوهشی علوم بالینی، مرکز تحقیقات بیماری های متابولیک، بیمارستان امام حسین، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: غلظت میکرو آراِن‌ای‌های سرمی با بروز بیماری‌های متابولیکی متعددی ازجمله چاقی، هایپرلیپیدمی و دیابت نوعِ دو، رابطه دارند. هدف کلی این پژوهش بررسی تغییرات mir146b متعاقب یک‌دوره مداخلات ورزشی در نوجوانان چاق یا دارای اضافه‌وزن است.
روش‌ها: در این مطالعه نیمه تجربی تعداد 30 دانش‌آموز پسر چاق و دارای اضافه‌وزن در بازه سنی13 تا 15 سال، بر اساس شاخص توده بدنی به شکل قابل دسترس انتخاب و در دو گروه تمرین‌های تناوبی شدید (شاخص توده بدنی 25/2±15/26 کیلوگرم/مترمربع) و مدرسه‌محور (شاخص توده بدنی 26/2±02/26 کیلوگرم/مترمربع) جای گرفتند. مداخلات تمرینی در طول 12 هفته و سه جلسه در هر هفته، پس از 10 تا 15 دقیقه گرم‌کردن اولیه انجام گردید و در پایان هر جلسه تمرینی 5 تا 10 دقیقه هم برای سردکردن بدن در نظر گرفته شد. برای استخراج miR146b گردش خون از دستگاه ریل تایم پی‌سی‌آر و برای اندازه‌گیری سطوح چربی از کیت‌های شرکت پارس آزمون و روش الایزا استفاده شد.
یافته‌ها: بیان miR146b در گروه تمرین‌های تناوبی شدید 88/46 درصد کاهش و در گروه تمرین‌های مدرسه‌محور نیز 05/62 درصد کاهش داشت، اما تفاوت معناداری بین دو گروه مشاهده نشد (836/0=p)، همچنین سطوح چربی در هر دو گروه بهبود یافت، اما در سطوح کلسترول (677/0=p)، تری‌گلیسرید (977/0=p)، LDL  (247/0=p) و HDL (977/0=p) تفاوت معناداری  بین گروه‌ها وجود نداشت.
نتیجه‌گیری: برای ایجاد تغییر در سطوح چربی و miR146b به‌عنوان نشانگر وضعیت پاتولوژیکی چاقی در کودکان و کاهش خطر ابتلا به انواع بیماری در بزرگسالی، می‌توان از هر دو تمرین مدرسه‌محور و تناوبی شدید استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Changes in miR146b Following Different Exercise Interventions in Adolescents with Overweight and Obesity

نویسندگان [English]

  • Rasol Khalil Tahmasebi 1
  • Vazgen Minasian 2
  • Silva Hovsepian 3

1 PhD Candidate, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran,

2 Associate professor, department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran

3 MD, PhD, of Clinical Sciences, Metabolic Liver Diseases Research Center, Imam Hossein Children's Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

چکیده [English]

Introduction: Serum microRNAs are associated with numerous metabolic diseases, including obesity, hyperlipidemia, and type 2 diabetes. This study aimed to identify the miR146b changes following different exercise training interventions in adolescents with overweight and obesity.
Methods: In this quasi-experimental study, 30 obese and overweight male adolescents aged 13-15 years were selected through the convenience sampling method based on body mass index (BMI) and assigned to two high-intensity interval training (HIIT) (Mean BMI: 26.15±2.25 kg/m2) and  school-based (SBE) (Mean BMI: 26.02±2.26 kg/m2) groups. Exercises in each group were performed for 12 weeks/ three weekly sessions after 10-15 minutes of initial warm-up. Moreover, at the end of each training session, 5-10 minutes were considered for cooling down. The circulating miR146b was extracted using a Real-Time PCR system. The subjects’ lipid profile was  measured by enzymatic methods, using certified commercial kits.
Results: The expression of miR146b in both training groups, HIIT and SBE, dropped around 46.88% vs. 62.05%, respectively, but there was no significant difference between groups (p=0.836). Lipid profile levels also improved in both HIIT and SBE groups, but there were no significant differences in the Cholesterol (p=0.677), Triglyceride (p=0.977), LDL (p=0.247), and HDL levels between the two groups (p=0.977).
Conclusion: The results showed that both school-based and high-intensity interval training can be used to modify lipid Profile and miR146b levels as an indicator of the pathological status of obesity in children and reduce the risk of disease in adulthood.

کلیدواژه‌ها [English]

  • Adolescent
  • Exercise
  • Interventions
  • miR146b
  • Obesity
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مجله علوم پزشکی صدرا
دوره 10، شماره 2
فروردین 1401
صفحه 97-110
فایل ها
هم رسانی
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