نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجو دکتری، گروه مهندسی شیمی، دانشکده مهندسی نیکبخت، دانشگاه سیستان و بلوچستان، زاهدان، ایران

2 دانشیار، عضو هیئت‌علمی، گروه مهندسی شیمی، دانشکده مهندسی نیکبخت، دانشگاه سیستان و بلوچستان، زاهدان، ایران

3 استاد، عضو هیئت‌علمی، گروه مهندسی شیمی، دانشکده مهندسی نیکبخت، دانشگاه سیستان و بلوچستان، زاهدان، ایران

4 استادیار، گروه کیفیت و ایمنی مواد غذایی، پژوهشکده علوم و صنایع غذایی، جهاد دانشگاهی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

مقدمه: یکی از دلایل اصلی عفونت‌های ادراری استفاده از کاتترهای ادراری می‌باشد. باگذشت زمان و استفاده بی‌رویه از آنتی‌بیوتیک‌های شیمیایی باکتری‌ها نسبت به درمان شیمیایی مقاوم شده‌اند؛ بنابراین بیشترین چالش بیمارستان‌ها جلوگیری از عفونت ادراری و جایگزین مناسب برای آنتی‌بیوتیک‌های شیمیایی می‌باشد. محققان بر روی گیاهان دارویی به‌عنوان جایگزین مناسب برای آنتی‌بیوتیک‌های شیمیایی پرداخته‌اند.
روش‌ها: در این پژوهش از عصاره گیاهی چای سبز و کاکوتی به‌عنوان عامل آنتی باکتریال برای آنتی باکتریال و آنتی بیوفیلم کردن کاتتر لاتکس پوشش داده‌شده با سیلیکون با روش تلقیح استفاده‌شده است. تست‌های دیسک دیفیوژن، نفوذ در براث، زاویه تماس، FE-SEM، AFM، ATR-FTIR، تست کشش انجام شد.
یافته‌ها: بعد از تلقیح تست دیسک دیفیوژن بر روی باکتری‌های اشرشیاکلی و استافیلوکوکوس اورئوس عامل عفونت ادراری انجام شد و خاصیت آنتی باکتریال کاتتر تائید شد. نتایج حاصل از تست نفوذ در براث طی مدت 21 روز (P≤0.0001) نشان‌دهنده کاهش باکتری‌ها طی گذر زمان بوده است. تست زاویه تماس افزایش خاصیت هیدروفیلیکی کاتتر ها بعد از اصلاح نشان داد (P≤0.0002). تست مکانیکی نشان‌دهنده افزایش مدول یانگ می‌باشد. تست SEM نشان کاهش چسبندگی باکتری بر سطح کاتتر است. تست AFM نشان از افزایش زبری بعد از اصلاح داد. وجود عصاره درون کاتترها به ATR-FTIR تائید شد.
نتیجه­ گیری: از نتایج حاصل می‌توان گفت گیاهان دارویی می‌تواند عامل مناسب برای تلقیح کاتترهای ادراری و کاهش عفونت ادراری در بیمارستان‌ها باشد توانایی از بین بردن باکتری‌ها را دارد. عصاره‌های گیاهی می‌توانند عامل مناسبی برای جایگزینی آنتی‌بیوتیک‌های شیمیایی باشند. همچنین عصاره‌ها خاصیت آب‌دوستی سطح را افزایش داده و مانع چسبندگی باکتری و خاصیت ضد بیوفیلیمی می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Improving the Antibacterial and Biofilm properties of Urinary Catheters by Using Green Tea and Ziziphora Extracts

نویسندگان [English]

  • Najme Akhlaghi-Ardekani 1
  • Davod Mohebbi-Kalhori 2
  • Abdolreza Samimi 3
  • Reza Karazhyan 4

1 Ph.D. student, Chemical Engineering Department, Nikbakht Faculty, University of Sistan and Baluchestan, Zahedan, Iran

2 Associate Professor, Faculty member, Chemical Engineering Department, Nikbakht Faculty, University of Sistan and Baluchestan, Zahedan, Iran

3 Professor, Faculty member, Chemical Engineering Department, Nikbakht Faculty, University of Sistan and Baluchestan, Zahedan, Iran

4 Assistant Professor, Food Industry Department, Jahad Daneshgahi Faculty, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده [English]

Introduction: One of the main causes of urinary infections is the use of urinary catheters. Over time, the overuse of chemical antibiotics has made urinary bacteria become resistant to chemical antibiotic treatments. Therefore, the major challenge in hospitals is preventing urinary infection and finding a suitable replacement for chemical antibiotics.  In recent years, researchers have been studying the use of herbal medicines to replace antibiotics.
Methods: In this study, green tea and ziziphora herbal extracts have been used as antibacterial agents for making silicone coated latex Foley catheters antibacterial and antibiofilm agents with inoculation. Various tests such as disc diffusion, broth penetration, contact angle, FE-SEM, AFM, ATR-FTIR, and elasticity were performed.
Results: After inoculation, disc diffusion test was performed on Staphylococcus aureus and Escherichia coli, urinary infection agents, and the antibacterial property of the silicone coated latex catheters was verified. In the penetration broth test, the herbal antibacterial catheters could eliminate the bacteria after 21 days (P˂0.0001). The contact angle test showed an increase in the hydrophilic property of the modified catheter (p˂0.0002). The mechanical test suggests an increase in Young module. SEM test indicates a decrease in bacteria adherence to the catheter surface. AFM test shows an increase in the roughness of the surface after impregnation. The presence of extracts in catheters was verified by ATR-FTIR.
Conclusion: It can be said from the obtained results that medicinal herbs can be appropriate agents for the inoculation of urinary catheters and the reduction of urinary infections in hospitals. In fact, modified catheters with herbal extracts could eliminate all bacteria. In addition, herbal extracts could be a good replacement for chemical antibiotics. Finally, herbal extracts could increase surface hydrophilicity,   prevent bacteria adherence, and they have antibiofilm properties.

کلیدواژه‌ها [English]

  • Antibacterial agent
  • Foley catheters
  • Herbal extract
  • Infectious bacteria
  • Urinary tract infection
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