Document Type : Original Article
Authors
1 Professor, Department of Biochemistry, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran
3 Assistant Professor, Department of Biochemistry, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
4 Professor, Department of Biochemistry, Pasteur Institute of Iran
Abstract
Introduction: In recent years, due to the spread of drug resistance in the Plasmodium parasite and the shortcomings of anti-malarial drugs, the use of liposome-based nanomedicines in treating malaria has garnered significant attention. This research studied the effect of liposomes containing the antimalarial compound Eosin B on the Plasmodium berghei parasite in murine malaria.
Methods: Liposomes containing Eosin B were prepared using cholesterol in the presence of Eosin B via solvent evaporation. Eosin B, Eosin B liposome, drug solvent, drug-free liposomes, and control drugs were administered to infected mice. The percentage of parasite inhibitory activity was evaluated using Peter’s test. In the in vitro model, the Plasmodium falciparum parasite was first cultured, and the survival percentage of the parasite in each group was investigated using the parasite lactate dehydrogenase test.
Results: In each test group, the percentage of parasitemia under the effect of the Eosin B liposome was lower than that of Eosin B alone, and the inhibition percentage was significantly higher (P<0.05). Also, the survival percentage of the parasite in the in vitro model was lower in each group under the effect of the Eosin B liposome compared to Eosin B alone.
Conclusion: Liposomes containing Eosin B can increase efficacy in the mouse malaria model and reduce the drug’s dose. Considering the reduction of drug dosage in treating malaria by the Eosin B liposome, using this liposome drug will be beneficial.
Keywords
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