Document Type : Original Article

Authors

1 Faculty Member of Islamic Azad University, Hidaj, Iran

2 Assistant Professor, Department of Physical Education, Faculty of Humanities and social sciences, Abhar Branch, Islamic Azad University, Abhar, Iran

3 Assistant Professor, Department of Physical Education, Faculty of Humanities and social sciences, Abhar Branch, Islamic Azad University, Zanjan, Iran

10.30476/smsj.2023.88611.1180

Abstract

Introduction: This study aimed to determine the effect of resistance, aerobic, and combined exercises on salivary malondialdehyde levels in active old men.
Methods: This semi-experimental study used a pre-test and post-test design with three training groups. A total of 36 subjects were randomly divided into three groups with 12 participants each (1. resistance training, 2. aerobic training, and 3. combined training). The salivary malondialdehyde value in subjects was evaluated and recorded using a spectrophotometer with a colorimetric method. The subjects then performed relevant training protocols for eight weeks, after which the post-test data were evaluated and recorded under conditions similar to the pre-test. Statistical analysis was performed using descriptive statistical tests, dependent t-test, one-way ANOVA, and Scheffe post-hoc in SPSS version 24. The significance level was set at 0.05. 
Results: The results showed that salivary malondialdehyde significantly increased after applying aerobic, resistance, and combined training (p < 0.001). This increase was 29.7%, 19.5%, and 11.1% for combined, resistance, and aerobic training, respectively.
Conclusion: According to this study’s findings, trainers and designers of elderly training programs should use combined training more frequently to benefit more from exercise’s antioxidant effects and improve total antioxidant capacity.
 

Keywords

  1. Sies H, Belousov VV, Chandel NS, Davies MJ, Jones DP, Mann GE, et al. Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology. Nat Rev Mol Cell Biol. 2022;23(7):499-515.
  2. Strzyz P. ATP and ROS signal cell extrusion. Nat Rev Mol Cell Biol. 2022;23(6):387.
  3. Naderifar H, Mohammad khani Gangeh M, Mehri F, Shamloo Kazemi S. Effects of high intensity interval training and consumption of matcha green tea on malondialdehyde and glutathione peroxidase levels in women. Journal of Mazandaran University of Medical Sciences. 2022;32(212):42-53. [in Persian].
  4. Mackinnon LT. Advances in exercise immunology. Champaign: Human Kinetics; 2007. p. 58-66.
  5. Song W, Tang Q, Teng L, Zhang M, Sha S, Li B, et al. Exercise for myocardial ischemia-reperfusion injury: A systematic review and meta-analysis based on preclinical studies. Microvasc Res. 2023;147:104502.
  6. Caldas LC, Salgueiro RB, Clarke ND, Tallis J, Barauna VG, Guimaraes-Ferreira L. Effect of Caffeine Ingestion on Indirect Markers of Exercise-Induced Muscle Damage: A Systematic Review of Human Trials. Nutrients. 2022;14(9).
  7. Bouzid MA, Hammouda O, Matran R, Robin S, Fabre C. Influence of physical fitness on antioxidant activity and malondialdehyde level in healthy older adults. Appl Physiol Nutr Metab. 2015;40(6):582-9.
  8. Yeh TS, Lei TH, Barnes MJ, Zhang L. Astragalosides Supplementation Enhances Intrinsic Muscle Repair Capacity Following Eccentric Exercise-Induced Injury. Nutrients. 2022;14(20).
  9. Nebl J, Drabert K, Haufe S, Wasserfurth P, Eigendorf J, Tegtbur U, et al. Exercise-Induced Oxidative Stress, Nitric Oxide and Plasma Amino Acid Profile in Recreational Runners with Vegetarian and Non-Vegetarian Dietary Patterns. Nutrients. 2019;11(8).
  10. Chen Y, Ji P, Ma G, Song Z, Tang BQ, Li T. Simultaneous determination of cellular adenosine nucleotides, malondialdehyde, and uric acid using HPLC. Biomed Chromatogr. 2021;35(10):e5156.
  11. Rangraz E, Mirzaei B, Rahmaninia F. The Effect of Resistance Training on Serum hs-CTnI and NT-proBNP Levels in Elderly Men. Journal of Health Promotion Management. 2019;7(6):17-24. [In Persian].
  12. Ghafari G, Bolboli L, Rajabi A, Saedmochshi S. The effect of 8 weeks aerobic training on predictive inflammatory markers of atherosclerosis and lipid profile in obese elderly women. Journal of Ilam University of Medical Sciences 2014; 23(7):144-154. [In Persian].
  13. Behmardi T, Banitalebi E, Ghafari M. Effects of Combined training strength and endurance on serum levels of homocysteine in elderly inactive woman. Journal of Geriatric Nursing. 2016;2(3):69-80. [In Persian].
  14. Barrera G, Pizzimenti S, Daga M, Dianzani C, Arcaro A, Cetrangolo GP, et al. Lipid Peroxidation-Derived Aldehydes, 4-Hydroxynonenal and Malondialdehyde in Aging-Related Disorders. Antioxidants (Basel). 2018;7(8).
  15. Morales M, Munné-Bosch S. Malondialdehyde: facts and artifacts. Plant physiology. 2019;180(3):1246-50.
  16. Ntovas P, Loumprinis N, Maniatakos P, Margaritidi L, Rahiotis C. The Effects of Physical Exercise on Saliva Composition: A Comprehensive Review. Dent J (Basel). 2022;10(1).
  17. Moslehi Najafabadi I, Debidi Roshan W, Fallah Mohammadi Z, PourAmir M. The effect of short-term vitamin E supplementation on the malondialdehyde response of healthy men following an exhausting exercise session at sea level and moderate altitude. Olympic Quarterly. 2017; 1 (41). [In Persian].
  18. Aghajani V, Nazari M, Shabani R. Impact of aerobic and resistance training supplemented with the consumption of saffron on glutathione peroxidase and malondialdehyde in men with type 2 diabetes. Journal of Gorgan University of Medical Sciences. 2019;21(3):24-33. [In Persian].
  19. Mohammadinejad S, Sayari AA, Moslehi M, Veysi Sheykhrobat M. Impact of Consumption of a Single Course of Probiotic Supplementation on Malondialdehyde, Total Antioxidant Capacity and Lactic Acid in Futsalist Women. Jundishapur Scientific Medical Journal. 2018;17(2):159-67. [In Persian].
  20. Moradi H, Nikbakht H, Ebrahim K, Natanzi HA. The effects of concurrent aerobic training and grape seed extract on total antioxidant capacity and malondialdehyde in young men. Medical Journal of Tabriz University of Medical Sciences. 2018;40(3):81-7. [In Persian].
  21. Amiri F, Babaie A, Satari M, Ghorbani M. Effect of Short-term Consumption of White Grape on Oxidative Stress by Measuring the Serum Malondialdehyde and Total Antioxidant Capacity Levels in Overweight. Journal of Mazandaran University of Medical Sciences. 2016;26(138):85-95. [In Persian].
  22. Sadeghi A, Gholami M. The Impact of Short-Term Garlic Supplements on Serum Total Antioxidant Capacity and Malondialdehyde in Active Men after Eccentric Exercise. Journal of Food Technology & Nutrition. 2018;15(1):57-64. [In Persian].
  23. Mirzaei B, Rahmani nia F, Rashidlami A, Ghahremani Moghaddam M. Comparison of effect of resistance exercise on blood total antioxidant capacity, bilirubin and uric acid between athlete and non-athlete elderly men. Metabolism and Exercise. 2014;3(2):129-39. [In Persian].
  24. Afzalpour MI, SaqibJo M, Zarban A, Jani M. Comparison of the effects of an acute resistance and aerobic exercise session on the antioxidant defense system and lipid peroxidation of healthy young men. Sports and Biological Sciences. 2011;3(2):30-9. [In Persian].
  25. Sies H, Jones DP. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nat Rev Mol Cell Biol. 2020;21(7):363-83.