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The Simultaneous Effect of Moderate-Intensity Continuous Aerobic Exercise and Rice Bran Extract on ABCG5 and ABCG8 Gene Expression in the Intestinal Tissue of Rats Fed with High-Fat Diet

Document Type : Original Article

Authors

1 Department of Sports Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Sports Physiology, Pardis Branch, Islamic Azad University, Pardis, Iran

10.30476/smsj.2025.103190.1554

Abstract

Introduction: Cholesterol homeostasis in the intestinal absorption pathway is regulated by the expression of various genes, such as ABCG5 and ABCG8. This study aimed to investigate the effects of moderate-intensity continuous aerobic exercise and rice bran extract on the expression of the ABCG5 and ABCG8 genes in the intestinal tissue of rats fed with a high-fat diet.
Methods: For this study, 30 eight-week-old female rats were randomly divided into five groups (n=6 per group), including 1) normal diet control, 2) high-fat diet control, 3) high-fat diet with aerobic exercise, 4) high-fat diet with rice bran extract, and 5) high-fat diet with combined aerobic exercise and rice bran extract. The moderate-intensity continuous aerobic exercise program was conducted over 6 weeks. Rice bran ethanol extract was administered via gavage at a dosage of 60 mg/Kg. The data were analyzed using SPSS software (version 26).
Results: ABCG5 and ABCG8 gene expression showed a significant increase in both the normal diet control group and the aerobic exercise group compared to the high-fat diet control group. The rice bran extract group also demonstrated elevated gene expression compared to the high-fat diet control. Furthermore, the combination of aerobic exercise and rice bran extract resulted in a significant increase in the expression of the studied genes compared to the high-fat diet control group.
Conclusion: The combination of moderate-intensity continuous aerobic exercise and rice bran extract enhanced the expression of ABCG5 and ABCG8 genes—key regulators of intestinal cholesterol absorption—more effectively than either intervention alone.

Keywords

References
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Sadra Medical Journal
Volume 13, Issue 3
October 2025
Pages 598-613
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