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The Effect of Resistance-Endurance Training on the Expression of IGF-1, Akt, and p70s6k Pathway Genes in the Gastrocnemius Muscle of Aged Rats

Document Type : Original Article

Authors

1 PhD Candidate in Exercise Physiology, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Professor in Exercise Physiology, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

3 Associate Professor in Exercise Physiology, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

10.30476/smsj.2025.101114.1475

Abstract

Introduction: Aging is a natural phenomenon accompanied by reduced physical activity and the onset of sarcopenia. Accordingly, this study aimed to investigate the effect of resistance-endurance training on the expression of IGF-1, Akt, and p70s6k pathway genes in the gastrocnemius muscle of aged rats.
Methods: In this experimental study, 14 male Wistar rats aged 22 to 24 months were randomly divided into control and resistance-endurance training groups. The training program was conducted for 10 weeks, with three sessions per week. Gene expression levels of IGF-1, Akt, and p70s6k pathway in skeletal muscle were measured using quantitative real-time PCR, and the data were analyzed using an independent t-test.
Results: The results showed that, compared to the control group, the resistance-endurance training group exhibited a significant increase in the expression of the IGF-1 gene (P=0.003), Akt (P=0.019), and p70s6k (P=0.004) genes after 10 weeks of training.
Conclusion: The findings suggested that resistance-endurance training probably increased the expression of IGF-1, Akt, and p70s6k pathway genes in aged rats. The activation of the Akt /p70s6k signaling pathway was partially induced by autocrine/paracrine response mediated by IGF-1, ultimately leading to muscle hypertrophy. These effects might also apply to humans. Therefore, further research on human samples is recommended.

Keywords

References
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Sadra Medical Journal
Volume 13, Issue 1
January 2025
Pages 66-78
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