Background: Shigella is responsible for shigellosis or bacillary dysentery, which is transmitted by contaminated food. Invasion Plasmid Antigens (Ipa) gene leads to intra- or extra-cellular spreading of Shigella. The current study aimed to identify Ipa and stx genes in Shigellasonnei isolated from clinical samples by multiplex-PCR and determine their antibiotic resistance profile. Methods: In this study, 150 stool samples were collected from different treatment centers in Tehran. After culture-based microbiological and biochemical tests, 60 Shigella sonnei species were identified. Antibiotic susceptibility tests were performed by disk diffusion method according to CLSI guideline. Then, multiplex-PCR assay was employed to identify stx and ipa genes. Results: The results showed that the highest and lowest resistance was related to co-trimoxazole (n=60, 100%) and ceftriaxone (n=10, 16.7%), respectively. Out of the 60 isolates, 57 strains (95%) were positive for ipaH gene and other virulence genes were not detected in any of the samples. Conclusion: ipaH, due to presence in the majority of Shigella strains, can be a candidate to identify this organism. It seems that indiscriminate use of antibiotics is one of the causes of antibiotic resistance in Shigella species.
Lee T-M, Chang L-L, Chang C-Y, Wang J-C, Pan T-M, Wang T-K, et al. Molecular analysis of Shigella sonnei isolated from three well-documented outbreaks in school children. J Med Microbiol 2000;49(4):355-60.
Lai V, Wang L, Reeves PR. Escherichia coli clone Sonnei (Shigella sonnei) had a chromosomal O-antigen gene cluster prior to gaining its current plasmid-borne O-antigen genes. J Bacteriol. 1998;180(11):2983-6.
Ghandian S, Sattari M, Nikbin VS, Aslani MM. Study of antibiotic susceptibility pattern and presence of ipaH gene among shigella strains isolated from selected provinces in Iran. Modares J Med Sci: Pathobiol 2011;14(1):81-8. (Persian).
Dutta S, Ghosh A, Ghosh K, Dutta D, Bhattacharya SK, Nair GB, et al. Newly emerged multiple-antibiotic-resistant Shigella dysenteriae type 1 strains in and around Kolkata, India, are clonal. J Clin Microbiol 2003;41(12):5833-4.
Nguyen TV, Van Le P, Le CH, Weintraub A. Antibiotic resistance in diarrheagenic Escherichia coli and Shigella strains isolated from children in Hanoi, Vietnam. Antimicrob Agents chemother 2005;49(2):816-9.
Obi C, Bessong P, Momba M, Potgieter N, Samie A, Igumbor E. Profiles of antibiotic susceptibilities of bacterial isolates and physico-chemical quality of water supply in rural Venda communities, South Africa. Water SA 2004;30(4):515-9.
Sur D, Ramamurthy T, Deen J, Bhattacharya S. Shigellosis: challenges & management issues. Indian J Med Res 2004;120(5):454.
Thapa B, Ventkateswarlu K, Malik A, Panigrahi D. Shigellosis in children from north India: a clinicopathological study. J Trop pediatrics 1995;41(5):303-7.
Venkatesan MM, Buysse JM, Kopecko DJ. Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella flexneri. Proc Natl Acad Sci 1988;85(23):9317-21.
Bourdet‐Sicard R, Rüdiger M, Jockusch BM, Gounon P, Sansonetti PJ, Van Nhieu GT. Binding of the Shigella protein IpaA to vinculin induces F‐actin depolymerization. The EMBO J 1999;18(21):5853-62.
Aranda K, Fagundes-Neto U, Scaletsky I. Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp. J Clinl Microbiol 2004;42(12):5849-53.
Girard MP, Steele D, Chaignat C-L, Kieny MP. A review of vaccine research and development: human enteric infections. Vaccine 2006;24(15):2732-50.
Sharma A, Singh SK, Bajpai D. Phenotypic and genotypic characterization of Shigella spp. with reference to its virulence genes and antibiogram analysis from river Narmada. Microbiol Res 2010;165(1):33-42.
Khorshidi A, Akbari H, Salehi A. prevalence of shigllosis, serotypes, antibiotic resistance pattern in the diarhogenic patients referred to kashan central laboratory in 2000-2001. Feyz Journals of Kashan University of Medical Sciences. 2006; 10(4): 65-70 (Persian).
Oh JY, Yu HS, Kim SK, Seol SY, Cho DT, Lee JC. Changes in patterns of antimicrobial susceptibility and integron carriage among Shigella sonnei isolates from southwestern Korea during epidemic periods. J Clin Microbiol 2003;41(1):421-3.
Ranjbar R, Aleo A, Giammanco GM, Dionisi AM, Sadeghifard N, Mammina C. Genetic relatedness among isolates of Shigella sonnei carrying class 2 integrons in Tehran, Iran, 2002–2003. BMC Infect Dis 2007;7(1):62.
Faruque SM, Khan R, Kamruzzaman M, Yamasaki S, Ahmad QS, Azim T, et al. Isolation of Shigella dysenteriae type 1 and S. flexneri strains from surface waters in Bangladesh: comparative molecular analysis of environmental Shigella isolates versus clinical strains. Appl Environ Microbiol 2002;68(8):3908-13.
Wayne P. Institute LS. Performance standards for antimicrobial disk susceptibility testing: approved standard: National Committee for Clinical Laboratory Standards 2012:1-37.
Qu M, Zhang X, Liu G, Huang Y, Jia L, Liang W, et al. An eight-year study of Shigella species in Beijing, China: serodiversity, virulence genes, and antimicrobial resistance. J Infect in Dev Countries 2014;8(07):904-8.
Farshad S, Ranjbar R, Hosseini M. Molecular Genotyping of Shigella sonnei Strains Isolated From Children With Bloody Diarrhea Using Pulsed Field Gel Electrophoresis on the Total Genome and PCR-RFLP of IpaH and IpaBCD Genes. Jundishapur J Microbiol 2015;8(1).
Salehi Chaleshtori A, Saadati M, Ahsani Arani Y, Bahador A, Hosseini M, Hesaraki M, et al. [Evaluation of Live Attenuated Shigella dysenteriae Type 1 Strain as a Candidate Vaccine]. Police Medicine 2012:1(1):27-35. (Persian).
MoezArdalan K, Zali MR, Dallal MMS, Hemami MR, Salmanzadeh-Ahrabi S. Prevalence and pattern of antimicrobial resistance of Shigella species among patients with acute diarrhoea in Karaj, Tehran, Iran. J Health, Population and Nutrition 2003:96-102 (Persian).
Scheutz F, Teel LD, Beutin L, Piérard D, Buvens G, Karch H, et al. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. J Clin Microbiol 2012;50(9):2951-63.
Lindsay B, Ochieng JB, Ikumapayi UN, Toure A, Ahmed D, Li S, et al. Quantitative PCR for detection of Shigella improves ascertainment of Shigella burden in children with moderate-to-severe diarrhea in low-income countries. J Clin Microbiol 2013;51(6):1740-6.
Ganjipour, M., Amini, K. & Moradli, G. (2016). Identification of Ipa and Stx Genes and Their Antibiotic Resistance Pattern in Shigella Sonnei isolated from Clinical Samples using Multiplex-PCR. Sadra Medical Journal, 4(2), 127–136.
MLA
Ganjipour, M., Amini, K., & Moradli, G. "Identification of Ipa and Stx Genes and Their Antibiotic Resistance Pattern in Shigella Sonnei isolated from Clinical Samples using Multiplex-PCR", Sadra Medical Journal, 4, 2, 2016, 127–136.
HARVARD
Ganjipour, M., Amini, K., & Moradli, G. (2016). 'Identification of Ipa and Stx Genes and Their Antibiotic Resistance Pattern in Shigella Sonnei isolated from Clinical Samples using Multiplex-PCR', Sadra Medical Journal, 4(2), pp. 127–136.
CHICAGO
Ganjipour, M., Amini, K. & Moradli, G., "Identification of Ipa and Stx Genes and Their Antibiotic Resistance Pattern in Shigella Sonnei isolated from Clinical Samples using Multiplex-PCR." Sadra Medical Journal, 4 2 (2016): 127–136,
VANCOUVER
Ganjipour M., Amini K., Moradli G. Identification of Ipa and Stx Genes and Their Antibiotic Resistance Pattern in Shigella Sonnei isolated from Clinical Samples using Multiplex-PCR. Sadra Medical Journal. 2016;4(2):127–136 (In Persian).
