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مداخله هم‌افزایی ورزش هوازی زیر بیشینه و مکمل روغن کتان بر بیان ژن هورمون‌های آدیپونکتین و امنتین-1 بافت چرب احشایی رت‌های نر ویستار چاق شده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد فیزیولوژی ورزش، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران

2 استاد فیزیولوژی کار و ورزش، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران

3 دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران

10.30476/smsj.2024.99571.1430

چکیده

مقدمه: امنتین-۱، آدیپوکاین جدیدی است که اغلب از بافت چربی احشایی ترشح شده و با چاقی ارتباط وارونه دارد. همچنین پروتئین اختصاصی آدیپوسیت آدیپونکتین، نقش برجسته در اثرات متابولیک چاقی ایفا می‌کند. هدف این مطالعه، مداخلات ورزش هوازی زیر بیشینه و مکمل روغن کتان، بر بیان ژن‌های هورمون آدیپونکتین و امنتین-۱ رت‌های چاق بود.
مواد و روش‌ها: تعداد 30 سر رت آزمایشگاهی در 5 گروه برابر شامل الف) گروه کنترل با مصرف غذای نرمال و چهار گروه با مصرف غذای پرچرب شامل ب) مکمل روغن کتان، ج) برنامه ورزش زیر بیشینه، د) ترکیب مداخله ورزشی و مکمل روغن کتان، هـ)گروه شم (رژیم غذای پرچرب) تقسیم شدند. برنامه تمرین هوازی دویدن با شدت معادل 55-70 درصد vo2max برای پنج روز در هفته و هشت هفته اجرا شد. مداخله روغن کتان یک گرم در هر کیلوگرم از وزن رت چاق نیز برای پنج روز در هفته و هشت هفته به رت‌ها خورانده شد.
یافته‌ها: مداخله برنامه ورزش به افزایش‌هایی در بیان ژن هورمون آدیپونکتین و امنتین-1 بافت چربی سفید احشایی رت‌های چاق انجامید (0/05>P) که با کاهش وزن رت‌ها رابطه مستقیمی داشت. اگرچه، مصرف مکمل روغن کتان تأثیر معناداری در بیان ژن هورمون آدیپونکتین داشت (0/05>P)، اما تغییری در بیان ژن امنتین-1 ایجاد نشد (0/05<P). این در حالی است که مداخله هم‌زمان متغیرهای مستقل (ورزش + مکمل روغن کتان) تأثیر معناداری در بیان ژن هر دو هورمون آدیپونکتین و امنتین-1 رت‌ها داشت (0/05>P).
نتیجه‌گیری: به نظر می‌رسد مداخله هم‌زمان تمرینات هوازی زیر بیشینه و مکمل روغن کتان تأثیر بارزتر از هر یک از دو متغیر مستقل بر افزایش بیان ژن آدیپونکتین و امنتین-1 دارد و احتمالاً به کنترل وزن بدن رت‌های چاق می‌انجامد.

کلیدواژه‌ها

عنوان مقاله [English]

The Synergistic Intervention of Submaximal Aerobic Exercise and Flaxseed Oil Supplementation on Gene Expression of Adiponectin and Omentin-1 Hormones in Visceral Adipose Tissue of Obese Male Wistar Rats

نویسندگان [English]

  • Mahmood Amani 1
  • Farzad Nazem 2
  • Hossein Saki 3

1 Master of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, Bu-Ali Sina University, Hamedan, Iran

2 Professor of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, Bu-Ali Sina University, Hamedan, Iran

3 PhD in sport physiology, Department of Physical Education and Sport Sciences, Bu-Ali Sina University, Hamedan, Iran

چکیده [English]

Introduction: Omentin-1 is a newly discovered adipokine often secreted from visceral adipose tissue and is inversely related to obesity. Additionally, the adipocyte-specific protein adiponectin plays a significant role in the metabolic effects of obesity. This study aimed to investigate the interventions of submaximal aerobic exercise and flax oil supplementation on the expression of adiponectin and omentin-1 hormone genes in obese rats.
Methods: Thirty laboratory rats were divided into five equal groups: a) a control group (with normal food consumption), and four groups with high-fat food consumption including b) linseed oil supplement, c) a submaximal exercise program, d) a combination of exercise intervention and flax oil supplement, and e) sham group (high-fat diet). An aerobic training program of running with an intensity equal to 55-70% vo2max was implemented five days a week for eight weeks. The intervention of linseed oil, one gram per kilogram of the weight of obese rats, was also administered to the rats five days a week for eight weeks.
Results: The exercise program intervention increased the gene expression of adiponectin and omentin-1 hormones in visceral white adipose tissue of obese rats (P<0.05). Flaxseed oil supplementation had a significant effect on the expression of the adiponectin hormone gene (P<0.05), but there was no change in the expression of the omentin-1 gene (P>0.05). However, the simultaneous intervention of independent variables significantly affected the gene expression of adiponectin and omentin-1 hormones in rats (P<0.05).
Conclusion: The simultaneous intervention of submaximal aerobic exercise and linseed oil supplementation has a more pronounced effect than either of the two independent variables on increasing the expression of adiponectin and omentin-1 genes, and it likely leads to the control of body weight in obese rats.

کلیدواژه‌ها [English]

  • Obesity
  • Rats
  • Exercise
  • Linseed oil
  • Omentin-1
  • Adiponectin
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مجله علوم پزشکی صدرا
دوره 12، شماره 3
تیر 1403
صفحه 330-340
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هم رسانی
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