نوع مقاله: مقاله مروری

نویسندگان

1 دانشیار بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران

2 دانشجوی دکترا بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران

3 دانشجوی فوق لیسانس بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران

10.30476/smsj.2019.79284.0

چکیده

پروتئین شیر، آلفا لاکتالبومین، یکی از پروتئین‌هایی است که مطالعات زیادی روی ساختار و عملکرد آن انجام‌شده است. در اواخر دهه 1990 فعالیت کاملاً متفاوتی از آن کشف شد. وقتی که آلفا لاکتالبومین درکانفورماسیونی قرار دارد که به‌طور نسبی تانخورده است می‌تواند با اولئیک اسید تشکیل مجموعه‌ای را دهد که به‌طور اختصاصی فعالیت ضد سرطانی دارد. مجموعه حاصل از آلفا لاکتالبومین انسانی و اسید اولئیک، HAMLET نامیده می‌شود. مکانیسم مرگ سلولی توسط HAMLET هنوز به‌طور دقیق مشخص نشده است، ولی نشان داده‌شده است که HAMLET با اندامک‌های مختلف سلولی ازجمله هسته، لیزوزم ها، میتوکندری، پروتئازوم ها وریبوزوم ها برهمکنش دارد. آپوپتوز، اتوفاژی و اختلال در ساختار کروماتین سه مسیر مختلف مسموم‌کننده‌ی سلولی هستند که در پاسخ به HAMLET فعال می‌شوند. در این مطالعه خلاصه ای از ساختار، مکانیسم‌های ضد سرطانی و کاربردهای بالینی این مجموعه ارائه می‌شود.
 

کلیدواژه‌ها

عنوان مقاله [English]

The Study of Clinical Application and Anticancer Mechanism of Alpha-lactalbumin and Oleic Acid Composition

نویسندگان [English]

  • Pooneh Mokarram 1
  • Hamid Behrouj 2
  • Sedigheh Rezayi 3
  • Morvarid Siri 3

1 Associate Professor of Clinical Biochemistry, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran

2 PhD Candidate in Clinical Biochemistry, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran

3 M.Sc. student of Clinical Biochemistry, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran

چکیده [English]

Milk protein, alpha-lactalbumin, is one of the proteins that many researchers have done a lot of research on its structure and function. In the late nineties, a completely different activity of α-LA was discovered. When α-LA is present in a conformation that has been partially unfolded, it can form a complex with oleic acid (OA), which specifically has an anti-cancer activity. The complex between human α-LA and OA has been called HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells). The cell death mechanism by HAMLET has not yet been determined. However, it has been shown that HAMLET interacts with various cellular organelles including nuclei, lysosomes, mitochondria, proteases, and ribosomes. Apoptosis, autophagy, and disorders in the structure of chromatin are three different cytotoxic pathways activated in response to HAMLET. In this study, a summary of the structure, antitumor mechanisms, and clinical applications of this complex will be presented.
 

کلیدواژه‌ها [English]

  • HAMLET
  • cancer
  • Oleic acid
  • Alpha lactalbumin
  • apoptosis

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